

Four different data sets were developed to evaluate Lys and Met requirements, rumen N balance, and milk yield predictions. In response to the feed-library changes and an increased supply of essential AA because of updated contents of AA, a combined efficiency of use was adopted in place of separate calculations for maintenance and lactation to better represent the biology of the cow. Degradation rates of protein and carbohydrate fractions were adjusted according to new fractionation schemes, and corresponding changes to equations used to calculate rumen outflows and postrumen digestion were presented. The changes to the feed library were significant and are reported in a separate manuscript. The objectives of this study were to describe updates to the CNCPS and evaluate model performance against both literature and on-farm data. New laboratory and animal sampling methods and data have been generated over the last 10 yr that had the potential to improve the predictions for energy, protein, and AA supply and requirements in the Cornell Net Carbohydrate and Protein System (CNCPS). In conclusion, feeding rumen-degradable valine increases plasma T4 and T3 concentrations, which would explain the mechanism leading to increased milk production. Concentrations of T3 (125.7, 140.7, 148.8, and 139.7 ng/dL) were increased for cows receiving rbST, V40, and V80 compared with CON cows, with cows receiving V40 having the highest T3 concentrations. Concentrations of T4 (3.28, 3.90, 3.98, and 3.87 µg/dL for CON, rbST, V40, and V80, respectively) were increased for cows receiving rbST, V40, and V80 compared with CON cows. Blood samples were collected twice during wk 2 and 3 of each period for T3 and T4 analysis. Cows were fed a total mixed ration with a distillers dried grains carrier fed at 113.4 g/d containing no valine or added valine. Treatments were control (CON), a single injection of recombinant bovine somatotropin (rbST), and synthetic valine fed at 40 (V40) or 80 (V80) g/cow per day.

Eight multiparous Holstein dairy cows (255 ± 26.4 d in milk) were blocked by milk yield (34.1 ± 8.25 kg/d) and randomly assigned to 1 of 4 treatments using a replicated 4 × 4 Latin square design with 21-d periods (7 d for dietary adaptation and 14 d for data collection). Previous short-term studies have demonstrated increased milk production when feeding iodinated casein. To further elucidate the response mechanism, we hypothesized that thyroxine (T4) and triiodothyronine (T3), which are important regulators of basal metabolism, may be involved.

Our previous published work demonstrated that feeding rumen-degradable valine to late-lactation dairy cows increased milk production compared with control-fed cows, with a response that was equivalent to that of recombinant bovine somatotropin.
